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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant function in advertising mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have already been explained. Two further splice variants ended up described but in depth nucleotide sequence information is not but available.
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Blocking peptides are peptides that bind particularly on the concentrate on antibody and block antibody binding. These peptide typically has the epitope recognized with the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the absolute best results are...
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This guide outlines additional feasible optimization of cellular and immunoassay parameters to make certain the very best success are...
Antibodies sure to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining with the blocked antibody as opposed to the antibody on your own, one can see which staining is particular.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is degraded as cells development through S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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